Fractionation of chicken egg proteome by isoelectric point in liquid phase
نویسندگان
چکیده
منابع مشابه
The isoelectric fractionation of hen's-egg ovotransferrin.
1. Hen ovotransferrin was examined by isoelectric fractionation. 2. The major component observed in starch-gel electrophoresis can be isolated from the minor component. 3. When non-saturating amounts of iron are added to ovotransferrin, isoelectric fractionation demonstrates the existence of three molecular species corresponding to the metal-free protein, the one-iron-atom-protein complex and t...
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Proteome-pI is an online database containing information about predicted isoelectric points for 5029 proteomes calculated using 18 methods. The isoelectric point, the pH at which a particular molecule carries no net electrical charge, is an important parameter for many analytical biochemistry and proteomics techniques, especially for 2D gel electrophoresis (2D-PAGE), capillary isoelectric focus...
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Due to the complexity of proteomes, developing methods of sample fractionation, separation, concentration, and detection have become urgent to the identification of large numbers of proteins, as well as the acquisition of those proteins in low abundance. In this work, liquid isoelectric focusing (LIEF) combined with 2D-LC-MS/MS was applied to the proteome of Saccharomyces cerevisiae. This yield...
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Pathogens are an important and significant hazard for human and animal health. In recent years, antibiotics are used to treat different types of infection caused by bacterial agents. In veterinary medicine, antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease, but the adverse effects of drugs have always been a major conc...
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In proteomic investigations, a number of different separation techniques can be applied to fractionate whole cell proteomes into more manageable fractions for subsequent analysis. In this work, utilizing HPLC and mass spectrometry for protein identification, two different fractionation methods were compared and contrasted to determine the potential of each method for the simple and reproducible...
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ژورنال
عنوان ژورنال: Acta Agraria Debreceniensis
سال: 2014
ISSN: 2416-1640,1587-1282
DOI: 10.34101/actaagrar/57/1957